Sodium selenite supplementation does not reduce the effectiveness of X-ray irradiation treatment on human cancerous esophageal TE-8 cells

Sodium selenite supplementation at a concentration 50 nM before X-ray irradiation was suggested to protect non-cancerous human esophageal CHEK-1 cells from irradiation-induced damage. This present study investigated those effects on cancerous cell line. The cancer line, TE-8, cultured and supplemented for the cytotoxicity assay, GPx-1 activity, viability clonogenic assay western blot analysis. An apoptosis biomarker, Cleaved PARP, used. results show that survival post-irradiation of supplemented-cells had same effect as treated by only, tended decrease (p=0.27), rate (p=1.00). cleaved PARP level higher in supplemented-and irradiated- than with alone. These suggest sodium prior does not reduce effectiveness treatment cells.